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Single-Cell RNA-Seq Analysis of Infiltrating Neoplastic Cells at the Migrating Front of Human Glioblastoma
Summary

This data set looked at the effects of the tumor on antigen-presenting cells within its main mass and proximal to it, as well as the molecular characteristics of infiltrating cells. Characterization of the effects of GBM (glioblastoma) on cells of the normal brain, and identifying the molecular characteristics of infiltrating GBM cells into the brain parenchyma. GBM samples came from four patients, with tissue from the tumor core and proximal tissue which was tumor-free, but within the vicinity of the tumor mass. The cells were dissociated, sorted, SMARTer cDNA synthesis in 96-well plates, then sequenced by Illumina with 75bp paired-end reads.


Experimetal Flowchart


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