This track displays the ENCODE Registry of candidate cis-Regulatory Elements (cCREs) in the mouse genome, a total of 339,815 elements identified and classified by the ENCODE Data Analysis Center according to biochemical signatures. cCREs are the subset of representative DNase hypersensitive sites across ENCODE samples that are supported by either histone modifications (H3K4me3 and H3K27ac) or CTCF-binding data. The Registry of cCREs is one of the core components of the integrative level of the ENCODE Encyclopedia of DNA Elements.
Additional exploration of the cCRE's and underlying raw ENCODE data is provided by the SCREEN (Search Candidate cis-Regulatory Elements) web tool, designed specifically for the Registry, accessible by linkouts from the track details page. The cCREs identified in the human genome are available in a companion track, here.
CCREs are colored and labeled according to classification by regulatory signature:
Color | UCSC label | ENCODE classification | ENCODE label | |
---|---|---|---|---|
red | prom | promoter-like signature | PLS | |
orange | enhP | proximal enhancer-like signature | pELS | |
yellow | enhD | distal enhancer-like signature | dELS | |
pink | K4m3 | DNase-H3K4me3 | DNase-H3K4me3 | |
blue | CTCF | CTCF-only | CTCF-only |
The DNase-H3K4me3 elements are those with promoter-like biochemical signature that are not within 200bp of an annotated TSS.
All individual DNase hypsersensitive sites (DHSs) identified from 176 DNase-seq profiles in mouse (a total of 26 million) were iteratively clustered and filtered for the highest signal across all experiments, producing 1.2 million representative DHSs (rDHSs). The highest signal elements from this set that were also supported by high H3K4me3, H3K27ac and/or CTCF ChIP-seq signals were designated cCRE's (a total of 339,815 in mouse).
Classification of cCRE's was performed based on the following criteria:
The GENCODE VM18 (Ensembl 93) basic gene annotation set was used in this analysis. For further detail about the identification and classification of ENCODE cCREs see the About page of the SCREEN web tool.
The ENCODE accession numbers of the constituent datasets at the ENCODE Portal are available from the cCRE details page.
The data in this track can be interactively explored with the Table Browser or the Data Integrator. The data can be accessed from scripts through our API, the track name is "encodeCcreCombined".
For automated download and analysis, this annotation is stored in a bigBed file that
can be downloaded from
our download server.
The file for this track is called encodeCcreCombined.bb.
Individual regions or the whole genome annotation can be obtained using our tool
bigBedToBed which can be compiled from the source code or downloaded as a precompiled
binary for your system.
Instructions for downloading source code and binaries can be found
here.
The tool can also be used to obtain only features within a given range, e.g.
bigBedToBed http://hgdownload.soe.ucsc.edu/gbdb/mm10/encode3/ccre/encodeCcreCombined.bb -chrom=chr21 -start=0 -end=100000000 stdout
This annotation is based on ENCODE data released on or before September 14, 2018.
This dataset was produced by the ENCODE Data Analysis Center (ZLab at UMass Medical Center). Please check the ZLab ENCODE Public Hubs for the most updated data. Thanks to Henry Pratt, Jill Moore, Michael Purcaro, and Zhiping Weng, PI for providing this data. Thanks also to the ENCODE Consortium, the ENCODE production laboratories, and the ENCODE Data Coordination Center for generating and processing the datasets used here.
ENCODE Project Consortium. Expanded Encyclopedias of DNA Elements in the Human and Mouse Genomes. Nature. 2020 July 30;583(7818):699-710
ENCODE Project Consortium. An integrated encyclopedia of DNA elements in the human genome. Nature. 2012 Sep 6;489(7414):57-74. PMID: 22955616; PMC: PMC3439153
ENCODE Project Consortium. A user's guide to the encyclopedia of DNA elements (ENCODE). PLoS Biol. 2011 Apr;9(4):e1001046. PMID: 21526222; PMC: PMC3079585