This directory contains the Oct. 2006 (AgamP3/anoGam3) assembly of the
A. gambiae genome (anoGam3, The International Consortium for the Sequencing of Anopheles Genome),
as well as repeat annotations and GenBank sequences.
For more information about this assembly, please note the NCBI resources:
https://www.ncbi.nlm.nih.gov/genome/46
https://www.ncbi.nlm.nih.gov/genome/assembly/305108
https://www.ncbi.nlm.nih.gov/bioproject/1438
https://www.ncbi.nlm.nih.gov/bioproject/SAMN02952903
Files included in this directory:
anoGam3.2bit - contains the complete A. gambiae/anoGam3 genome sequence
in the 2bit file format. Repeats from RepeatMasker and Tandem Repeats
Finder (with period of 12 or less) are shown in lower case; non-repeating
sequence is shown in upper case. The utility program, twoBitToFa (available
from the kent src tree), can be used to extract .fa file(s) from
this file. A pre-compiled version of the command line tool can be
found at:
http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86_64/
See also:
http://genome.ucsc.edu/admin/git.html
http://genome.ucsc.edu/admin/jk-install.html
anoGam3.chrom.sizes - Two-column tab-separated text file containing assembly
sequence names and sizes.
anoGam3.agp.gz - Description of how the assembly was generated from
fragments.
anoGam3.fa.gz - "Soft-masked" assembly sequence in one file.
Repeats from RepeatMasker and Tandem Repeats Finder (with period
of 12 or less) are shown in lower case; non-repeating sequence is
shown in upper case.
anoGam3.fa.masked.gz - "Hard-masked" assembly sequence in one file.
Repeats are masked by capital Ns; non-repeating sequence is shown in
upper case.
anoGam3.fa.out.gz - RepeatMasker .out file. RepeatMasker was run with the
-s (sensitive) setting.
January 31 2015 (open-4-0-5) version of RepeatMasker
RepBase library: RELEASE 20140131
anoGam3.trf.bed.gz - Tandem Repeats Finder locations, filtered to keep repeats
with period less than or equal to 12, and translated into UCSC's BED
format.
est.fa.gz - A. gambiae ESTs in GenBank. This sequence data is updated once a
week via automatic GenBank updates.
ucscToFlyBase.lift - lift file to translate UCSC coordinates to
FlyBase/Ensembl genomes coordinates. Use this file with the kent
command line tool: 'liftUp' which can be obtained from:
http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86_64/
md5sum.txt - checksums of files in this directory
mrna.fa.gz - A. gambiae mRNA from GenBank. This sequence data is updated
once a week via automatic GenBank updates.
xenoMrna.fa.gz - GenBank mRNAs from species other than that of
the genome. This sequence data is updated once a week via automatic
GenBank updates.
anoGam3.gc5Base.wigVarStep.gz - ascii data wiggle variable step values used
- to construct the GC Percent track
anoGam3.gc5Base.wig.gz - wiggle database table for the GC Percent track
- this is an older standard alternative to the current
- bigWig format of the track, sometimes usefull for analysis
anoGam3.gc5Base.wib - binary data to correspond with the gc5Base.wig file
see also: http://genome.ucsc.edu/goldenPath/help/wiggle.html
and http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database
for a discussion of how to use the wig.gz and .wib files for
interaction with the GC percent data values
anoGam3.chromAlias.txt - sequence name alias file, one line
for each sequence name. First column is sequence name followed by
tab separated alias names.
------------------------------------------------------------------
If you plan to download a large file or multiple files from this
directory, we recommend that you use ftp rather than downloading the
files via our website. To do so, ftp to hgdownload.soe.ucsc.edu
[username: anonymous, password: your email address], then cd to the
directory goldenPath/anoGam3/bigZips. To download multiple files, use
the "mget" command:
mget <filename1> <filename2> ...
- or -
mget -a (to download all the files in the directory)
Alternate methods to ftp access.
Using an rsync command to download the entire directory:
rsync -avzP rsync://hgdownload.soe.ucsc.edu/goldenPath/anoGam3/bigZips/ .
For a single file, e.g. chromFa.tar.gz
rsync -avzP
rsync://hgdownload.soe.ucsc.edu/goldenPath/anoGam3/bigZips/chromFa.tar.gz .
Or with wget, all files:
wget --timestamping
'ftp://hgdownload.soe.ucsc.edu/goldenPath/anoGam3/bigZips/*'
With wget, a single file:
wget --timestamping
'ftp://hgdownload.soe.ucsc.edu/goldenPath/anoGam3/bigZips/chromFa.tar.gz'
-O chromFa.tar.gz
To unpack the *.tar.gz files:
tar xvzf <file>.tar.gz
To uncompress the fa.gz files:
gunzip <file>.fa.gz
-----------------------------------------------------------------------------
GenBank Data Usage
The GenBank database is designed to provide and encourage access within
the scientific community to the most up to date and comprehensive DNA
sequence information. Therefore, NCBI places no restrictions on the use
or distribution of the GenBank data. However, some submitters may claim
patent, copyright, or other intellectual property rights in all or a
portion of the data they have submitted. NCBI is not in a position to
assess the validity of such claims, and therefore cannot provide comment
or unrestricted permission concerning the use, copying, or distribution
of the information contained in GenBank.
-----------------------------------------------------------------------------
Name Last modified Size Description
Parent Directory -
genes/ 2020-02-05 13:46 -
refMrna.fa.gz 2019-10-14 20:30 20
est.fa.gz.md5 2019-10-14 20:29 44
mrna.fa.gz.md5 2019-10-14 20:15 45
refMrna.fa.gz.md5 2019-10-14 20:30 48
xenoMrna.fa.gz.md5 2019-10-14 20:25 49
xenoRefMrna.fa.gz.md5 2019-10-14 20:30 52
md5sum.txt 2019-01-17 15:50 586
anoGam3.agp.gz 2017-12-21 08:26 85K
anoGam3.chrom.sizes 2017-12-19 15:10 182K
ucscToFlyBase.lift 2017-12-20 11:13 362K
anoGam3.chromAlias.txt 2022-09-08 14:07 471K
anoGam3.trf.bed.gz 2017-12-21 08:27 652K
anoGam3.gc5Base.wig.gz 2019-01-17 14:43 1.1M
anoGam3.chromAlias.bb 2022-09-08 14:07 2.4M
anoGam3.fa.out.gz 2017-12-21 08:27 6.7M
mrna.fa.gz 2019-10-14 20:15 16M
est.fa.gz 2019-10-14 20:29 31M
anoGam3.gc5Base.wib 2019-01-17 14:43 49M
anoGam3.2bit 2017-12-19 22:03 65M
anoGam3.fa.masked.gz 2017-12-21 08:29 67M
anoGam3.fa.gz 2017-12-21 08:28 78M
anoGam3.gc5Base.wigVarStep.gz 2017-12-19 15:20 127M
xenoRefMrna.fa.gz 2019-10-14 20:30 331M
xenoMrna.fa.gz 2019-10-14 20:25 6.8G