# Assembly name: ASM5464341v1 # Organism name: Pelophylax nigromaculatus (dark-spotted frog) # Isolate: GW-2025 # Sex: male # Taxid: 8409 # BioSample: SAMN51991534 # BioProject: PRJNA1335551 # Submitter: Hunan Agricultural University # Date: 2026-01-22 # Assembly type: haploid # Release type: major # Assembly level: Chromosome # Genome representation: full # WGS project: JBRIJY01 # Assembly method: hifiasm v. 0.15.1-r334; MECAT2 v. 2.0.0; NextDenovo v. 2.4.0 # Expected final version: Yes # Genome coverage: 60.0x # Sequencing technology: PacBio HiFi; Illumina; Hi-C # RefSeq category: Reference Genome # GenBank assembly accession: GCA_054643415.1 # ## Assembly-Units: ## GenBank Unit Accession RefSeq Unit Accession Assembly-Unit name ## GCA_054643425.1 Primary Assembly # # Ordered by chromosome/plasmid; the chromosomes/plasmids are followed by # unlocalized scaffolds. # Unplaced scaffolds are listed at the end. # RefSeq is equal or derived from GenBank object. # # Sequence-Name Sequence-Role Assigned-Molecule Assigned-Molecule-Location/Type GenBank-Accn Relationship RefSeq-Accn Assembly-Unit Sequence-Length UCSC-style-name chr1 assembled-molecule 1 Chromosome CM138083.1 <> na Primary Assembly 783679812 na chr2 assembled-molecule 2 Chromosome CM138084.1 <> na Primary Assembly 609529028 na chr3 assembled-molecule 3 Chromosome CM138085.1 <> na Primary Assembly 585672757 na chr4 assembled-molecule 4 Chromosome CM138086.1 <> na Primary Assembly 576860057 na chr5 assembled-molecule 5 Chromosome CM138087.1 <> na Primary Assembly 472610646 na chr6 assembled-molecule 6 Chromosome CM138088.1 <> na Primary Assembly 293703751 na chr7 assembled-molecule 7 Chromosome CM138089.1 <> na Primary Assembly 270424181 na chr8 assembled-molecule 8 Chromosome CM138090.1 <> na Primary Assembly 263052642 na chr9 assembled-molecule 9 Chromosome CM138091.1 <> na Primary Assembly 232988753 na chr10 assembled-molecule 10 Chromosome CM138092.1 <> na Primary Assembly 221991352 na chr11 assembled-molecule 11 Chromosome CM138093.1 <> na Primary Assembly 198892081 na chr12 assembled-molecule 12 Chromosome CM138094.1 <> na Primary Assembly 197716657 na chr13 assembled-molecule 13 Chromosome CM138095.1 <> na Primary Assembly 177440065 na unanchor1 unplaced-scaffold na na JBRIJY010000014.1 <> na Primary Assembly 13694861 na unanchor2 unplaced-scaffold na na JBRIJY010000015.1 <> na Primary Assembly 8868260 na unanchor3 unplaced-scaffold na na JBRIJY010000016.1 <> na Primary Assembly 7894433 na unanchor4 unplaced-scaffold na na JBRIJY010000017.1 <> na Primary Assembly 6412120 na unanchor5 unplaced-scaffold na na JBRIJY010000018.1 <> na Primary Assembly 5909144 na unanchor6 unplaced-scaffold na na JBRIJY010000019.1 <> na Primary Assembly 5746086 na unanchor7 unplaced-scaffold na na JBRIJY010000020.1 <> na Primary Assembly 5447222 na unanchor8 unplaced-scaffold na na JBRIJY010000021.1 <> na Primary Assembly 4989943 na unanchor9 unplaced-scaffold na na JBRIJY010000022.1 <> na Primary Assembly 4626193 na unanchor10 unplaced-scaffold na na JBRIJY010000023.1 <> na Primary Assembly 4212250 na unanchor13 unplaced-scaffold na na JBRIJY010000024.1 <> na Primary Assembly 3712625 na unanchor14 unplaced-scaffold na na JBRIJY010000025.1 <> na Primary Assembly 3604052 na unanchor15 unplaced-scaffold na na JBRIJY010000026.1 <> na Primary Assembly 3273390 na unanchor17 unplaced-scaffold na na JBRIJY010000027.1 <> na Primary Assembly 3095565 na unanchor19 unplaced-scaffold na na JBRIJY010000028.1 <> na Primary Assembly 2613691 na unanchor20 unplaced-scaffold na na JBRIJY010000029.1 <> na Primary Assembly 2573760 na unanchor21 unplaced-scaffold na na JBRIJY010000030.1 <> na Primary Assembly 2536718 na unanchor22 unplaced-scaffold na na JBRIJY010000031.1 <> na Primary Assembly 2439995 na unanchor25 unplaced-scaffold na na JBRIJY010000032.1 <> na Primary Assembly 2349765 na unanchor26 unplaced-scaffold na na JBRIJY010000033.1 <> na Primary Assembly 2331695 na unanchor27 unplaced-scaffold na na JBRIJY010000034.1 <> na Primary Assembly 2244209 na unanchor28 unplaced-scaffold na na JBRIJY010000035.1 <> na Primary Assembly 2202771 na unanchor29 unplaced-scaffold na na JBRIJY010000036.1 <> na Primary Assembly 2171762 na unanchor32 unplaced-scaffold na na JBRIJY010000037.1 <> na Primary Assembly 2071868 na unanchor34 unplaced-scaffold na na JBRIJY010000038.1 <> na Primary Assembly 1904538 na unanchor36 unplaced-scaffold na na JBRIJY010000039.1 <> na Primary Assembly 1891715 na unanchor37 unplaced-scaffold na na JBRIJY010000040.1 <> na Primary Assembly 1851331 na unanchor38 unplaced-scaffold na na JBRIJY010000041.1 <> na Primary Assembly 1823586 na unanchor39 unplaced-scaffold na na JBRIJY010000042.1 <> na Primary Assembly 1760573 na unanchor40 unplaced-scaffold na na JBRIJY010000043.1 <> na Primary Assembly 1756198 na unanchor42 unplaced-scaffold na na JBRIJY010000044.1 <> na Primary Assembly 1739310 na unanchor43 unplaced-scaffold na na JBRIJY010000045.1 <> na Primary Assembly 1672268 na unanchor45 unplaced-scaffold na na JBRIJY010000046.1 <> na Primary Assembly 1594344 na unanchor47 unplaced-scaffold na na JBRIJY010000047.1 <> na Primary Assembly 1573046 na unanchor49 unplaced-scaffold na na JBRIJY010000048.1 <> na Primary Assembly 1535190 na unanchor51 unplaced-scaffold na na JBRIJY010000049.1 <> na Primary Assembly 1489404 na unanchor55 unplaced-scaffold na na JBRIJY010000050.1 <> na Primary Assembly 1459416 na unanchor57 unplaced-scaffold na na JBRIJY010000051.1 <> na Primary Assembly 1442023 na unanchor58 unplaced-scaffold na na JBRIJY010000052.1 <> na Primary Assembly 1435101 na unanchor59 unplaced-scaffold na na JBRIJY010000053.1 <> na Primary Assembly 1433631 na unanchor61 unplaced-scaffold na na JBRIJY010000054.1 <> na Primary Assembly 1396593 na unanchor62 unplaced-scaffold na na JBRIJY010000055.1 <> na Primary Assembly 1341577 na unanchor67 unplaced-scaffold na na JBRIJY010000056.1 <> na Primary Assembly 1259569 na unanchor68 unplaced-scaffold na na JBRIJY010000057.1 <> na Primary Assembly 1258100 na unanchor69 unplaced-scaffold na na JBRIJY010000058.1 <> na Primary Assembly 1226738 na unanchor71 unplaced-scaffold na na JBRIJY010000059.1 <> na Primary Assembly 1187967 na unanchor76 unplaced-scaffold na na JBRIJY010000060.1 <> na Primary Assembly 1155362 na unanchor77 unplaced-scaffold na na JBRIJY010000061.1 <> na Primary Assembly 1137304 na unanchor78 unplaced-scaffold na na JBRIJY010000062.1 <> na Primary Assembly 1105107 na unanchor84 unplaced-scaffold na na JBRIJY010000063.1 <> na Primary Assembly 1073397 na unanchor90 unplaced-scaffold na na JBRIJY010000064.1 <> na Primary Assembly 1022400 na unanchor91 unplaced-scaffold na na JBRIJY010000065.1 <> na Primary Assembly 1016540 na unanchor94 unplaced-scaffold na na JBRIJY010000066.1 <> na Primary Assembly 995862 na unanchor95 unplaced-scaffold na na JBRIJY010000067.1 <> na Primary Assembly 987978 na unanchor101 unplaced-scaffold na na JBRIJY010000068.1 <> na Primary Assembly 964413 na unanchor102 unplaced-scaffold na na JBRIJY010000069.1 <> na Primary Assembly 960450 na unanchor103 unplaced-scaffold na na JBRIJY010000070.1 <> na Primary Assembly 957743 na unanchor112 unplaced-scaffold na na JBRIJY010000071.1 <> na Primary Assembly 867263 na unanchor114 unplaced-scaffold na na JBRIJY010000072.1 <> na Primary Assembly 854842 na unanchor116 unplaced-scaffold na na JBRIJY010000073.1 <> na Primary Assembly 847052 na unanchor117 unplaced-scaffold na na JBRIJY010000074.1 <> na Primary Assembly 841307 na unanchor120 unplaced-scaffold na na JBRIJY010000075.1 <> na Primary Assembly 828252 na unanchor122 unplaced-scaffold na na JBRIJY010000076.1 <> na Primary Assembly 804285 na unanchor123 unplaced-scaffold na na JBRIJY010000077.1 <> na Primary Assembly 798806 na unanchor128 unplaced-scaffold na na JBRIJY010000078.1 <> na Primary Assembly 766526 na unanchor131 unplaced-scaffold na na JBRIJY010000079.1 <> na Primary Assembly 734476 na unanchor140 unplaced-scaffold na na JBRIJY010000080.1 <> na Primary Assembly 664320 na unanchor142 unplaced-scaffold na na JBRIJY010000081.1 <> na Primary Assembly 653346 na unanchor144 unplaced-scaffold na na JBRIJY010000082.1 <> na Primary Assembly 651529 na unanchor145 unplaced-scaffold na na JBRIJY010000083.1 <> na Primary Assembly 649713 na unanchor146 unplaced-scaffold na na JBRIJY010000084.1 <> na Primary Assembly 631073 na unanchor164 unplaced-scaffold na na JBRIJY010000085.1 <> na Primary Assembly 575414 na unanchor173 unplaced-scaffold na na JBRIJY010000086.1 <> na Primary Assembly 525568 na unanchor175 unplaced-scaffold na na JBRIJY010000087.1 <> na Primary Assembly 523707 na unanchor181 unplaced-scaffold na na JBRIJY010000088.1 <> na Primary Assembly 503684 na unanchor200 unplaced-scaffold na na JBRIJY010000089.1 <> na Primary Assembly 446328 na unanchor201 unplaced-scaffold na na JBRIJY010000090.1 <> na Primary Assembly 446035 na unanchor202 unplaced-scaffold na na JBRIJY010000091.1 <> na Primary Assembly 440473 na unanchor210 unplaced-scaffold na na JBRIJY010000092.1 <> na Primary Assembly 432383 na unanchor211 unplaced-scaffold na na JBRIJY010000093.1 <> na Primary Assembly 427650 na unanchor222 unplaced-scaffold na na JBRIJY010000094.1 <> na Primary Assembly 390763 na unanchor223 unplaced-scaffold na na JBRIJY010000095.1 <> na Primary Assembly 388361 na unanchor226 unplaced-scaffold na na JBRIJY010000096.1 <> na Primary Assembly 383506 na unanchor232 unplaced-scaffold na na JBRIJY010000097.1 <> na Primary Assembly 375833 na unanchor233 unplaced-scaffold na na JBRIJY010000098.1 <> na Primary Assembly 373926 na unanchor239 unplaced-scaffold na na JBRIJY010000099.1 <> na Primary Assembly 361657 na unanchor241 unplaced-scaffold na na JBRIJY010000100.1 <> na Primary Assembly 354906 na unanchor246 unplaced-scaffold na na JBRIJY010000101.1 <> na Primary Assembly 346822 na unanchor247 unplaced-scaffold na na JBRIJY010000102.1 <> na Primary Assembly 344904 na unanchor256 unplaced-scaffold na na JBRIJY010000103.1 <> na Primary Assembly 334679 na unanchor258 unplaced-scaffold na na JBRIJY010000104.1 <> na Primary Assembly 328711 na unanchor276 unplaced-scaffold na na JBRIJY010000105.1 <> na Primary Assembly 282894 na unanchor277 unplaced-scaffold na na JBRIJY010000106.1 <> na Primary Assembly 282203 na unanchor285 unplaced-scaffold na na JBRIJY010000107.1 <> na Primary Assembly 257982 na unanchor286 unplaced-scaffold na na JBRIJY010000108.1 <> na Primary Assembly 257792 na unanchor287 unplaced-scaffold na na JBRIJY010000109.1 <> na Primary Assembly 257722 na unanchor288 unplaced-scaffold na na JBRIJY010000110.1 <> na Primary Assembly 257557 na unanchor294 unplaced-scaffold na na JBRIJY010000111.1 <> na Primary Assembly 249604 na unanchor296 unplaced-scaffold na na JBRIJY010000112.1 <> na Primary Assembly 248181 na unanchor301 unplaced-scaffold na na JBRIJY010000113.1 <> na Primary Assembly 242968 na unanchor304 unplaced-scaffold na na JBRIJY010000114.1 <> na Primary Assembly 239340 na unanchor305 unplaced-scaffold na na JBRIJY010000115.1 <> na Primary Assembly 239164 na unanchor327 unplaced-scaffold na na JBRIJY010000116.1 <> na Primary Assembly 204280 na unanchor328 unplaced-scaffold na na JBRIJY010000117.1 <> na Primary Assembly 203485 na unanchor334 unplaced-scaffold na na JBRIJY010000118.1 <> na Primary Assembly 197506 na unanchor344 unplaced-scaffold na na JBRIJY010000119.1 <> na Primary Assembly 186753 na unanchor356 unplaced-scaffold na na JBRIJY010000120.1 <> na Primary Assembly 180734 na unanchor370 unplaced-scaffold na na JBRIJY010000121.1 <> na Primary Assembly 168662 na unanchor375 unplaced-scaffold na na JBRIJY010000122.1 <> na Primary Assembly 166143 na unanchor376 unplaced-scaffold na na JBRIJY010000123.1 <> na Primary Assembly 164697 na unanchor381 unplaced-scaffold na na JBRIJY010000124.1 <> na Primary Assembly 163271 na unanchor400 unplaced-scaffold na na JBRIJY010000125.1 <> na Primary Assembly 154980 na unanchor420 unplaced-scaffold na na JBRIJY010000126.1 <> na Primary Assembly 149256 na unanchor431 unplaced-scaffold na na JBRIJY010000127.1 <> na Primary Assembly 144592 na unanchor435 unplaced-scaffold na na JBRIJY010000128.1 <> na Primary Assembly 143198 na unanchor453 unplaced-scaffold na na JBRIJY010000129.1 <> na Primary Assembly 133438 na unanchor458 unplaced-scaffold na na JBRIJY010000130.1 <> na Primary Assembly 130790 na unanchor470 unplaced-scaffold na na JBRIJY010000131.1 <> na Primary Assembly 127267 na unanchor474 unplaced-scaffold na na JBRIJY010000132.1 <> na Primary Assembly 124995 na unanchor480 unplaced-scaffold na na JBRIJY010000133.1 <> na Primary Assembly 124050 na unanchor492 unplaced-scaffold na na JBRIJY010000134.1 <> na Primary Assembly 120359 na unanchor508 unplaced-scaffold na na JBRIJY010000135.1 <> na Primary Assembly 114947 na unanchor510 unplaced-scaffold na na JBRIJY010000136.1 <> na Primary Assembly 114184 na unanchor531 unplaced-scaffold na na JBRIJY010000137.1 <> na Primary Assembly 103451 na unanchor540 unplaced-scaffold na na JBRIJY010000138.1 <> na Primary Assembly 101299 na unanchor546 unplaced-scaffold na na JBRIJY010000139.1 <> na Primary Assembly 99851 na unanchor571 unplaced-scaffold na na JBRIJY010000140.1 <> na Primary Assembly 91435 na unanchor591 unplaced-scaffold na na JBRIJY010000141.1 <> na Primary Assembly 87349 na unanchor609 unplaced-scaffold na na JBRIJY010000142.1 <> na Primary Assembly 84565 na unanchor611 unplaced-scaffold na na JBRIJY010000143.1 <> na Primary Assembly 83770 na unanchor621 unplaced-scaffold na na JBRIJY010000144.1 <> na Primary Assembly 81373 na unanchor627 unplaced-scaffold na na JBRIJY010000145.1 <> na Primary Assembly 80493 na unanchor630 unplaced-scaffold na na JBRIJY010000146.1 <> na Primary Assembly 79534 na unanchor647 unplaced-scaffold na na JBRIJY010000147.1 <> na Primary Assembly 76158 na unanchor648 unplaced-scaffold na na JBRIJY010000148.1 <> na Primary Assembly 76084 na unanchor651 unplaced-scaffold na na JBRIJY010000149.1 <> na Primary Assembly 74930 na unanchor656 unplaced-scaffold na na JBRIJY010000150.1 <> na Primary Assembly 73887 na unanchor663 unplaced-scaffold na na JBRIJY010000151.1 <> na Primary Assembly 72464 na unanchor684 unplaced-scaffold na na JBRIJY010000152.1 <> na Primary Assembly 69441 na unanchor693 unplaced-scaffold na na JBRIJY010000153.1 <> na Primary Assembly 68210 na unanchor708 unplaced-scaffold na na JBRIJY010000154.1 <> na Primary Assembly 65938 na unanchor716 unplaced-scaffold na na JBRIJY010000155.1 <> na Primary Assembly 65535 na unanchor727 unplaced-scaffold na na JBRIJY010000156.1 <> na Primary Assembly 64521 na unanchor730 unplaced-scaffold na na JBRIJY010000157.1 <> na Primary Assembly 64382 na unanchor736 unplaced-scaffold na na JBRIJY010000158.1 <> na Primary Assembly 63227 na unanchor740 unplaced-scaffold na na JBRIJY010000159.1 <> na Primary Assembly 62369 na unanchor767 unplaced-scaffold na na JBRIJY010000160.1 <> na Primary Assembly 57006 na unanchor790 unplaced-scaffold na na JBRIJY010000161.1 <> na Primary Assembly 54710 na unanchor796 unplaced-scaffold na na JBRIJY010000162.1 <> na Primary Assembly 54087 na unanchor800 unplaced-scaffold na na JBRIJY010000163.1 <> na Primary Assembly 53821 na unanchor803 unplaced-scaffold na na JBRIJY010000164.1 <> na Primary Assembly 53789 na unanchor806 unplaced-scaffold na na JBRIJY010000165.1 <> na Primary Assembly 53313 na unanchor813 unplaced-scaffold na na JBRIJY010000166.1 <> na Primary Assembly 52730 na unanchor832 unplaced-scaffold na na JBRIJY010000167.1 <> na Primary Assembly 50546 na unanchor845 unplaced-scaffold na na JBRIJY010000168.1 <> na Primary Assembly 49995 na unanchor849 unplaced-scaffold na na JBRIJY010000169.1 <> na Primary Assembly 49995 na unanchor855 unplaced-scaffold na na JBRIJY010000170.1 <> na Primary Assembly 49995 na unanchor901 unplaced-scaffold na na JBRIJY010000171.1 <> na Primary Assembly 45676 na unanchor937 unplaced-scaffold na na JBRIJY010000172.1 <> na Primary Assembly 41887 na unanchor947 unplaced-scaffold na na JBRIJY010000173.1 <> na Primary Assembly 41019 na unanchor966 unplaced-scaffold na na JBRIJY010000174.1 <> na Primary Assembly 39327 na unanchor979 unplaced-scaffold na na JBRIJY010000175.1 <> na Primary Assembly 38007 na unanchor981 unplaced-scaffold na na JBRIJY010000176.1 <> na Primary Assembly 37610 na unanchor982 unplaced-scaffold na na JBRIJY010000177.1 <> na Primary Assembly 37561 na unanchor1008 unplaced-scaffold na na JBRIJY010000178.1 <> na Primary Assembly 35482 na unanchor1038 unplaced-scaffold na na JBRIJY010000179.1 <> na Primary Assembly 33306 na unanchor1095 unplaced-scaffold na na JBRIJY010000180.1 <> na Primary Assembly 28582 na