Description

This track shows regions of this target genome (white-tufted-ear marmoset - 2021-04-28 - Vertebrate Genomes Project) that has alignment to other query genomes ("chain" subtracks) or in synteny ("net" subtracks). The alignable parts are shown with thick blocks that look like exons. Non-alignable parts between these are shown like introns.

Other query genome assemblies aligning to this target genome assembly:

• % 72.634 GCA_028885655.2_NHGRI_mPonAbe1-v2.0_pri Sumatran orangutan 2024-01-05 National Human Genome Research Institute, National Institutes of Health
• % 72.630 GCA_028885625.2_NHGRI_mPonPyg2-v2.0_pri Bornean orangutan 2024-01-08 National Human Genome Research Institute, National Institutes of Health
• % 72.402 GCA_029281585.2_NHGRI_mGorGor1-v2.0_pri western lowland gorilla 2024-01-08 National Human Genome Research Institute, National Institutes of Health
• % 72.352 GCA_029289425.2_NHGRI_mPanPan1-v2.0_pri pygmy chimpanzee 2024-01-08 National Human Genome Research Institute, National Institutes of Health
• % 72.345 GCA_028858775.2_NHGRI_mPanTro3-v2.0_pri chimpanzee 2024-01-08 National Human Genome Research Institute, National Institutes of Health
• % 72.231 hs1 Human Jan. 2022 (T2T CHM13v2.0/hs1) Telomere to telomere (T2T) assembly of haploid CHM13 + chrY (GCA_009914755.4)
• % 72.216 hg38 Human Dec. 2013 (GRCh38/hg38) GRCh38 Genome Reference Consortium Human Reference 38 (GCA_000001405.15)
• % 70.694 GCA_028878055.2_NHGRI_mSymSyn1-v2.0_pri siamang 2024-01-05 National Human Genome Research Institute, National Institutes of Health
• % 25.826 GCF_020740605.2_mLemCat1.pri Ring-tailed lemur 2021-11-04 Vertebrate Genomes Project
• % 12.290 GCF_027406575.1_mNycCou1.pri slow loris 2022-12-28 Vertebrate Genomes Project

Alignments identity

Chain Track

The chain tracks shows alignments of the other genome assemblies to the white-tufted-ear marmoset//hive/data/genomes/asmHubs/refseqBuild/GCF/011/100/555/GCF_011100555.1_mCalJa1.2.pat.X/html/GCF_011100555.1_mCalJa1.2.pat.X.names.tab/GCF_011100555.1/2021-04-28 genome using a gap scoring system that allows longer gaps than traditional affine gap scoring systems. It can also tolerate gaps in both query and target genomes simultaneously. These "double-sided" gaps can be caused by local inversions and overlapping deletions in both species.

The chain track displays boxes joined together by either single or double lines. The boxes represent aligning regions. Single lines indicate gaps that are largely due to a deletion in the query assembly or an insertion in the target assembly. Double lines represent more complex gaps that involve substantial sequence in both species. This may result from inversions, overlapping deletions, an abundance of local mutation, or an unsequenced gap in one species. In cases where multiple chains align over a particular region of the target genome, the chains with single-lined gaps are often due to processed pseudogenes, while chains with double-lined gaps are more often due to paralogs and unprocessed pseudogenes.

In the "pack" and "full" display modes, the individual feature names indicate the chromosome, strand, and location (in thousands) of the match for each matching alignment.

There could be four different types of chain tracks:

• Chains - The first level of chain track showing all potential chains. The other chain tracks are derived from this chain data.
• Syntenic - Filtered first level chain showing the corresponding regions between the two genomes in the alignment that have the same order of blocks and direction in the alignment.
• Reciprocal best - Filtered first level chain showing the corresponding regions where the best target to query alignment, and the best query to target alignment identify the same regions.
• Lift over - filtered first level chain selecting out the best/longest syntenic regions used to translate coordinates from the target genome to the query genome.

Alignment Track

The alignment track shows the net derived from the chain data in the format of a pair-wise side by side alignment. The net file is converted to the MAF format for this display.

Display Conventions and Configuration

Chain Track

By default, the chains to chromosome-based assemblies are colored based on which chromosome they map to in the aligning organism. To turn off the coloring, check the "off" button next to: Color track based on chromosome.

To display only the chains of one chromosome in the aligning organism, enter the name of that chromosome (e.g. chr4) in box next to: Filter by chromosome.

Alignment Track

At base level in full display mode, this track will show the sequence of query as it aligned to target. When the view is too large to show such detail, blocks of alignments will show corresponding alignments to other chromosomes with colors indicating other chromosomes.

Methods

Chain track

The query genome was aligned to target genome with lastz. The resulting alignments were converted into axt format using the lavToAxt program. The axt alignments were fed into axtChain, which organizes all alignments between a single query chromosome and a single target chromosome into a group and creates a kd-tree out of the gapless subsections (blocks) of the alignments. A dynamic program was then run over the kd-trees to find the maximally scoring chains of these blocks.

Alignment track

Chains were derived from lastz alignments, using the methods described on the chain tracks description pages, and sorted with the highest-scoring chains in the genome ranked first. The program chainNet was then used to place the chains one at a time, trimming them as necessary to fit into sections not already covered by a higher-scoring chain. During this process, a natural hierarchy emerged in which a chain that filled a gap in a higher-scoring chain was placed underneath that chain. The program netSyntenic was used to fill in information about the relationship between higher- and lower-level chains, such as whether a lower-level chain was syntenic or inverted relative to the higher-level chain. The program netClass was then used to fill in how much of the gaps and chains contained Ns (sequencing gaps) in one or both species and how much was filled with transposons inserted before and after the two organisms diverged.

The resulting net file was converted to axt format via netToAxt, then converted to maf format via axtToMaf, then converted to the bigMaf format with mafToBigMaf and bedToBigBed

Credits

lastz was developed by Robert Harris, Pennsylvania State University.

The axtChain program was developed at the University of California at Santa Cruz by Jim Kent with advice from Webb Miller and David Haussler.

The browser display and database storage of the chains and nets were created by Robert Baertsch and Jim Kent.

The chainNet, netSyntenic, and netClass programs were developed at the University of California Santa Cruz by Jim Kent.

References

Harris, R.S. (2007) Improved pairwise alignment of genomic DNA Ph.D. Thesis, The Pennsylvania State University

Chiaromonte F, Yap VB, Miller W. Scoring pairwise genomic sequence alignments. Pac Symp Biocomput. 2002:115-26. PMID: 11928468

Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D. Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9. PMID: 14500911; PMC: PMC208784

Schwartz S, Kent WJ, Smit A, Zhang Z, Baertsch R, Hardison RC, Haussler D, Miller W. Human-mouse alignments with BLASTZ. Genome Res. 2003 Jan;13(1):103-7. PMID: 12529312; PMC: PMC430961